Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For isolation and enumeration of anaerobic bacteria.
Composition
| Ingredients | g / L |
|---|---|
| Tryptone | 5.670 |
| Proteose peptone | 5.000 |
| Soya peptone | 1.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 5.830 |
| Sodium chloride | 1.670 |
| Dipotassium hydrogen phosphate | 0.830 |
| Tris (hydroxymethyl) aminomethane | 3.000 |
| L-Cystine | 0.400 |
| Hemin | 0.010 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Schaedler Agar was originally formulated by Schaedler et al (1) and further modified by Mata et al (2) with formulation changes (3) for cultivation and enumeration of aerobic and anaerobic microorganisms. Schaedler Agar supplemented with Vitamin K1 and 5% sheep blood is used for the recovery of fastidious anaerobic bacteria such as Bacteroides. Inclusion of Colistin and Nalidixic acid in the formulation (Schaedler CNA Agar) along with 5% sheep blood is used for the selective isolation of the anaerobic gram-positive cocci (4), Peptococcus and Peptostreptococcus species. Inclusion of Kanamycin and Vancomycin in the formulation (Schaedler KV Agar) along with 5% sheep blood is used for selective isolation of gram- negative anaerobes.Schaedler Agar serve as an excellent basal media to which blood or other enrichments can be added to enhance the recovery of fastidious anaerobic organisms.
The combination of tryptone, proteose peptone and Soya peptone, Yeast extract and L-cystine provide nitrogenous growth factors, vitamins and other essential growth nutrients. Dextrose serves as energy source. Hemin and sheep blood stimulates the growth of fastidious microorganisms and stimulates growth of other Bacteroides species and gram-positive spore formers (5). Addition of Sodium Polyanethol Sulphonate (SPS) is recommended when using this medium for blood culture (6). It inhibits phagocytosis and neutralizes the antibacterial activity of fresh blood components (7,8). Vitamin K1 enables the cultivation of Bacteroides melaninogenicus (9) and stimulates growth of other Bacteroides species and gram-positive spore formers (5).
Type of specimen
Clinical samples - Genital specimen, Upper respiratory swab
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (10,11). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Schaedler Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Light amber coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.40-7.80
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours under anaerobic condition.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacteroides fragilis ATCC 25285 | 50-100 | luxuriant | >=50% |
| Clostridium butyricum ATCC 13732 | 50-100 | luxuriant | >=50% |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant | >=50% |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant | >=50% |
| Escherichia coli ATCC 25922 (00013*) | >=103 | inhibited | 0% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=50% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).
| Product Name | Schaedler Agar Plate |
|---|---|
| SKU | MP291 |
| Customized Product Available | No |
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