Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended use
Recommended for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc. It can also be used to enumerate the organisms from water insoluble products & fatty products containing preservatives or antimicrobials.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Yeast extract | 6.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Sodium pyruvate | 2.000 |
| Histidine | 1.000 |
| Sodium thiosulphate | 0.050 |
| Glycerol | 10.00ml |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Soyabean Casein Digest Agar w/ Sodium pyruvate, Yeast extract, LTHTh & 0.5% Glycerol is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, products containing antimicrobials or preservatives (1) Tryptone, soya peptone, yeast extract and sodium pyruvate provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for microbial replication. Glycerol is additional source of carbon. Lecithin, polysorbate 80 (Tween 80) and thiosulphate act as neutralizing agents reported to neutralize the activity of antimicrobial agents. Lecithin and polysorbate 80 neutralizes quaternary ammonium compounds and parahydroxy benzoates. Sodium thiosulphate neutralizes mercurial, halogens, aldehydes etc. Histidine acts as a reducing agent.
Type of specimen
Environmental monitoring samples
Specimen Collection and Handling
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (2).
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay.
- Environmental Monitoring Test: Exposure of media plates for 4 h as a settle plate or in air sampler or even under laminar air flow may lead reduction in some available moisture on the surface. This may cause development of tiny cracks in the agar or slight shrinkage. This however, does not impact the performance of the media.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- It is recommended to store the plates at 24-30°C to avoid minimum condensation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar Plate w/ Sodium Pyruvate, Yeast extract & LTHTH & 1% Glycerol in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles. (y-irradiated, Triple pack)
Colour
Light yellow coloured medium.
Quantity of Medium
32ml of medium in 90 mm disposable plates
pH
7.10-7.50
Dose of irradiation (Kgy)
13.00-20.00
Sterility Check
Passes release criteria.
Growth Promotion Test
Growth Promotion was carried out in accordance with the harmonized method of USP/EP/BP/JP, and growth was observed after an incubation at 30-35°C for <=3 days for bacteria and <=5 days for fungus. Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35° C for <=18 hours).
Neutralization Test
Passes release criteria The smaller zone of inhibition compared to SCDA indicates neutralization of quaternary ammonium compounds
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Growth promoting | ||||||
| **Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| $ Kokuria rhizophila ATCC 9341 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| Clostridium sporogenes ATCC 19404 (00008*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=48 hours |
Key : (*) Corresponding WDCM numbers,
($) Formerly known as Micrococcus luteus,
(**) Formerly known as Bacillus subtilis subsp. spizizenii, (#)Formerly known as Aspergillus niger,
(^) Formerly known as Pseudomonas aeruginosa.
Storage and Shelf Life
On receipt store between 20-30°C.Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | SCDA Plate w/sodium pyruvate, yeast extract & LTHTh & 1% glycerol (γ-irradiated)(Triple pack) |
|---|---|
| SKU | MP2112AGT |
| Customized Product Available | No |
Write Your Own Review