Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for isolation and confirmation of Listeria species based on haemolysis and CAMP test from food samples. The composition and performance criteria of this medium are as per the specifications laid down in ISO 11290-2-2017(E).
Composition
ISO 11290-1 Specification - Blood Agar
| Ingredients | Gms / Litre |
|---|---|
| Enzymatic digest of animal tissues | 15.000 |
| Liver digest | 2.500 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 9 g to 18 g |
| Sheep Blood | 50ml |
| Final pH (at 25°C) | 7.2±0.2 |
MP2096I- Blood Agar Plate for Listeria
| Ingredients | Gms/Litre |
|---|---|
| Peptone # | 15.000 |
| HML extract ## | 2.500 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Sheep Blood | 50ml |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Enzymatic digest of animal tissues
## - Equivalent to Liver digest
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
L. monocytogenes is a gram positive, facultatively anaerobic rod shaped bacteria. It can grow under refrigerated condition and therefore is a major concern to the food industry. The recovery of Listeria is very low from food and environmental samples, hence it requires enrichment and then further isolation. Various selective and differential media have been proposed for the detection of Listeria species in particular L. monocytogenes (1).
Blood Agar Base is recommended by ISO (2,3) for the isolation of Listeria species from food, animal feeds and environmental samples in areas of food production and food handling. This medium contains sterile defibrinated sheep blood helps in the detection of haemolytic pattern. It differentiates between pathogenic Listeria species, beta haemolytic which includes L.monocytogenes, L.seeligeri and L.ivanovii from non-beta haemolytic and non-pathogenic species which include L.innocua, L.grayi and L.welshimeri. This plates can also be used to perform CAMP test, on basis of which inter species can be differentiated. The CAMP test is performed using S.aureus and Rhodococcus equi.Other strains of Listeria such as L.fleischmanii, L.marthii, L.rocourtiae and L.weihenstephanensis show negative beta haemolytic reaction and negative reaction is observed when CAMP test is performed with S. aureus and R. equi.
Peptone, HML extract and yeast extract supplies nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients required by the organisms. Sodium chloride maintains osmotic balance.
Type of specimen
Pure isolates from food and animal feeds, environmental samples in the area of food production and food handling.
Specimen Collection and Handling
Processing: (3)
Initial Suspension: According to sample type, prepare initial suspension in an appropriate diluent.
Surface plating: Surface plating on HiCrome™™ Listeria Ottaviani-Agosti Agar Plate (M1540I) of a specified quantity of the test sample for liquid products or of the initial suspension for other products and/or decimal dilutions if required.
Incubation: Incubation of the Petri dishes at 37 °C and examination after 24 h and after a further 24 h.
Confirmation: Confirmation of presumptive colonies of L. monocytogenes or Listeria spp. by means of appropriate morphological or biochemical tests i.e Hemolysis and CAMP Test.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Blood Agar for Listeria in 90mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles.
Colour: Cherry Red coloured medium
Quantity of medium: 25ml of medium in disposable plate
SterilityCheck: Passes release criteria
pH: 7.00-7.40
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. (Further incubate until satisfactory growth is observed)
| Organism | Inoculum (CFU) | Growth | Recovery | beta-haemolysis | CAMP test w/ S.aureus | CAMP test w/ R.equi |
|---|---|---|---|---|---|---|
| Listeria monocytogenes ATCC 35152 (00109*) | 50-100 | good-luxuriant | >=70% | positive | positive | negative |
| Listeria monocytogenes ATCC 19112 | 50-100 | good-luxuriant | >=70% | positive | positive | negative |
| Listeria monocytogenes ATCC 13932 (00021*) | 50-100 | good-luxuriant | >=70% | positive | positive | negative |
| Listeria monocytogenes ATCC 19111 (00020*) | 50-100 | good-luxuriant | >=70% | positive | positive | negative |
| Listeria ivanovii ATCC 19119 | 50-100 | good-luxuriant | >=70% | positive | negative | positive |
| Listeria innocua ATCC 33090 (00017*) | 50-100 | good-luxuriant | >=70% | negative | negative | negative |
| Listeria seeligeri ATCC 35967 | 50-100 | good-luxuriant | >=70% | weak positive | weak positive | weak positive |
| Listeria welshimeri ATCC 43549 | 50-100 | good-luxuriant | >=70% | negative | negative | negative |
| Listeria grayi ATCC 19120 | 50-100 | good-luxuriant | >=70% | negative | negative | negative |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Blood Agar Plate for Listeria |
|---|---|
| SKU | MP2096I |
| Customized Product Available | No |
Write Your Own Review