Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic fibrosis and other non-clinical specimens.
Composition**
| Ingredients | g/L |
|---|---|
| Casitose # | 10.000 |
| Lactose | 10.000 |
| Sucrose | 10.000 |
| Sodium chloride | 5.000 |
| Yeast extract | 1.500 |
| Phenol red | 0.080 |
| Crystal violet | 0.002 |
| Gentamicin | 0.010 |
| Vancomycin | 0.0025 |
| Polymyxin B | 600000 units |
| Agar | 14.000 |
| Final pH (at 25°C) | 7.0±0.1 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Casein peptone
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Burkholderia cepacia is an important opportunistic pathogen and causes pulmonary infection among individuals with cystic fibrosis (CF). Burkholderia cepacia species are gram negative, rod shaped bacteria. The organism may lead to Burkholderia cepacia syndrome, a neutralizing pneumonia associated with fever that culminates in to a rapid and fatal clinical deterioration (1). Burkholderia cepacia species may cause severe infection in individuals with cystic fibrosis and immunosuppressed individuals. B. cepacia is difficult to isolate on routinely used laboratory media like MacConkey Agar, since B.cepacia is a slow grower and therefore it is usually outgrown by the faster growing Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Burkholderia Cepacia Agar is based on PC medium, which was originally devised by Gilligan (2). This medium was found to be superior to MacConkey Agar for growth of B. cepacia. Burkholderia cepacia have the potential of overcoming antimicrobial preservative systems and antiseptics, and can grow in preserved aqueous oral liquids and topical products. This medium is recommended for detection of Burkholderia cepacia in pharmaceutical products.
Casitose and yeast extract in the medium provides the carbonaceous, nitrogenous, long chain amino acids, vitamin B source and other essential nutrients. Crystal violet and antimicrobial agents are used as selective agents. Crystal violet and vancomycin inhibits gram-positive cocci including Enterococci and Staphylococci. The antibiotics namely polymyxin B and gentamicin inhibits gram-negative bacteria.
B. cepacia metabolizes pyruvate forming alkaline end products. Sucrose and Lactose are the fermentable carbohydrate. The phenol red indicator changes colour from pink orange to pink red in alkaline pH.
Test procedure: The sample is initially enriched in Soyabean Casein Digest Medium and then plated on Burkholderia Cepacia Selective Agar.
Type of specimen
Clinical samples: Respiratory secretions
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Other bacteria resistant to the selective agents may grow on this media.
- Further biochemical characterization is necessary for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Burkholderia cepacia Selective Agar in 90 mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles
Colour of medium
Orange coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
6.90-7.10
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 30-35°C for 18-72 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony (CFU) |
|---|---|---|---|---|
| Burkholderia cepacia ATCC 25416 | 50-100 | good-luxuriant | >=50% | greenish brown colonies w/ yellow halo or white colonies surrounded by pink zone |
| Burkholderia cenocepacia ATCC 25608 | 50-100 | good-luxuriant | >=50% | greenish brown colonies w/ yellow halo or white colonies surrounded by pink zone |
| Burkholderia cenocepacia ATCC BAA-245 | 50-100 | good-luxuriant | >=50% | greenish brown colonies w/ yellow halo or white colonies surrounded by pink zone |
| Burkholderia multivorans ATCC BAA-247 | 50-100 | good-luxuriant | >=50% | greenish brown colonies w/ yellow halo or white colonies surrounded by pink zone |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | >=103 | inhibited | 0% | |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 0% |
Key: (*) corresponding WDCM numbers
^ Formerly known as Pseudomonas aeruginosa
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Burkholderia cepacia Selective Agar Plate |
|---|---|
| SKU | MP2089 |
| Customized Product Available | No |
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