Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for rapid isolation and identification of Methicillin Resistant Staphylococcus aureus (MRSA) from clinical specimens.
Composition
| Ingredients | g/L |
|---|---|
| Special peptone | 20.000 |
| Casitose | 20.000 |
| Sodium chloride | 8.500 |
| Carbohydrate | 14.000 |
| Phenol red | 0.025 |
| Chromogenic mix | 6.500 |
| Amino-Vitamin mix | 1.200 |
| Agar | 15.000 |
| ACC Selective Supplement (FD319) | |
| Cefoxitin | 10.000 mg |
| Colistin | 10.000 mg |
| Amphotericin B | 10.000 mg |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
▲Equivalent to Casein peptone
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
MRSA is a resistant variation of the common bacterium Staphylococcus aureus. It is an invasive pathogen that can cause disease in almost any tissue or organ in the human body, primarily in compromised individuals (1). Staphylococcal infections were earlier treated using Penicillin. But over the years resistance to this drug developed. Methicillin was the next drug of choice. While methicillin is very effective in treating most Staphylococcus infections some strains have developed resistance to methicillin and can no longer be killed by this antibiotic. These resistant bacteria are called Methicillin Resistant Staphylococcus aureus (MRSA) (2). Patients with breaks in their skin due to wound, indwelling catheters or burns are those with certain risk of developing MRSA infection (3).
Special peptone, Casitose and amino-vitamin mix provides essential nutrients for growth. Carbohydrate is the source of carbon and energy. Phenol red is the pH indicator. The chromogenic mixture incorporated in the medium is specifically cleaved by Staphylococcus aureus (MRSA) to give greenish yellow coloured colonies. Sodium chloride in the medium helps to maintain the osmotic equilibrium of the medium. High concentration of sodium chloride also helps in inhibiting the accompanying microflora. Agar acts as solidifying agent.
Type of specimen
Clinical samples - Tissue samples, wound swab, nasal swab, skin lesions, etc.
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Certain strains of MRSA which are intermediate may show poor growth. Further incubation upto 48 hours should be carried out.
- Further sensitivity can be carried out to ascertain the extent of resistance.
- Other methicillin resistant Staphylococcus species may grow. Further biochemical tests must be carried out to differentiate between resistant strains.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile HiCrome™ Rapid MRSA Agar in 90mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles
Colour of medium: Reddish orange coloured medium
Quantity of medium: 25ml of medium in disposable plate
pH: 7.20-7.60
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after an incubation at 30-35°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Staphylococcus aureus, MRSA ATCC 43300 | 50-100 | luxuriant | >=50% | greenish yellow (Note: Green colour may develop after 48 hours) |
| Staphylococcus epidermidis, MRSE | 50-100 | luxuriant | >=50% | blue |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=103 | inhibited | 0% | |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=103 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | >=103 | inhibited | 0% | |
| Candida albicans ATCC 10231 (00054*) | >=103 | inhibited | 0% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Hicrome™ Rapid MRSA Agar Plate |
|---|---|
| SKU | MP1974 |
| Customized Product Available | No |
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