Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for isolation and presumptive identification of Actinobacillus actinomycetemcomitans.
Composition**
| Ingredients | g / L |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Yeast extract | 1.000 |
| Agar | 15.000 |
| TSBV Supplement (FD323) | 1vial |
| Bacitracin | 75.000 mg |
| Vancomycin | 5.000 mg |
| Horse Serum (RM1239) | 100.000ml |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Tryptone Soya Serum Bacitracin Vancomycin Agar is enriched media recommended for the selective isolation and identification of Actinobacillus actinomycetemcomitans by J.Slots(1). TSBV agar are used in oral microbiological studies.(2). The detection rate for A.actinomycetemcomitans in the adult group is 67% with severe periodontitis, it suggests that this bacterium is important not only in localized juvenile peri-odontitis but also in periodontitis in adults (3). Tryptone and Soya peptone provide amino acids and other complex nitrogenous substances. Dextrose is the energy source. Dipotassium hydrogen phosphate buffers the medium. Yeast extract is the rich source of vitamin B complex. The medium is enriched with Horse serum for the good growth of A.actinomycetemcomitans. Bacitracin and Vancomycin inhibits most gram-positive and gram-negative anaerobes.
Type of specimen
Clinical samples - Tooth sample or gum tissue sample
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Tryptone Soya Serum Bacitracin Vancomycin Agar (TSBV) in 90mm disposable plate with smooth surface and absence of black particles/cracks/bubbles..
Colour of medium: Light yellow coloured medium
Quantity of medium: 25 ml of medium in 90 mm disposable plates.
pH: 6.90-7.30
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics after 48 hours at 35-37°C when incubated anaerobically.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Actinobacillus actinomycetemcomitans | 50-100 | good-luxuriant | >=50% |
| Fusobacterium nucleatum | 50-100 | good-luxuriant | >=50% |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | |
| Clostridium difficile ATCC 11204 | >=104 | inhibited |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Tryptone Soya Serum Bacitracin Vancomycin Agar (TSBV) Plate |
|---|---|
| SKU | MP1948 |
| Customized Product Available | No |
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