Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the detection of Extended Spectrum B-Lactamase-producing organisms from clinical & environmental samples.
Composition
| Ingredients | Amount |
|---|---|
| Peptone mix | 12.000 g/L |
| Sodium chloride | 4.000 g/L |
| Chromogenic mixture | 5.000 g/L |
| Buffer mix | 4.000 g/L |
| Agar | 15.000 g/L |
| AC3F Selective Supplement (FD278) | 2 vials |
| Ceftazidime (2x1.50mg) | 3.000 mg |
| Cefotaxime (2x1.50mg) | 3.000 mg |
| Ceftriazone (2x1.00mg) | 2.000 mg |
| Aztreonam (2x1.00 mg) | 2.000 mg |
| Fluconazole (2x 5.00 mg) | 10.0000 mg |
| Final pH (at 25°C) | 6.8±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Extended-spectrum ß-lactamase (ESBL)-producing organisms are an increasing challenge for healthcare practitioners fighting healthcare-associated infections (HAIs). Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca are the most common ESBL-producing pathogens (1). ESBL-producing organisms are generally resistant to many classes of antibiotics, including aminoglycosides and fluoroquinolones; ESBL-producing organisms are able to attack newer cephems and monobactams as well as narrow-spectrum cephalosporins and antigram-negative penicillins (2). They are associated with increased mortality and are difficult to detect and treat. The widespread use of extended-spectrum, third-generation cephalosporins, introduced in the 1980's to treat antibiotic-resistant bacteria, is believed to be a major contributor to the emergence of ESBL-producing organisms.
HiCrome™ ESBL Agar Base is chromogenic screening medium for the selective isolation of ESBL producing organisms. It contains peptone mix which serves as the carbon and nitrogen sources, long chain amino acids, vitamins and other growth nutrients. Chromogenic mixture is used to differentiate the ESBL producing organisms on the basis of colour. AC3F Selective Supplement (FD278) helps in inhibition of other contaminating organisms. ESBL producing E.coli grow as either pink or purple colonies.
ESBL producing members of the KESC group produce bluish green colonies; Proteus, Morganella and Providencia do not utilize any chromogen resulting in colourless to light brown colonies.
This medium can be inoculated with liquid suspension equivalent to 0.5 McFarland turbidity, prepared from rectal screening swabs, faecal samples or from isolated colony. Isolated colonies should not be directly plated on to this medium, because the high level inoculum may cause false positive results. Further confirmation using biochemical identification tests is recommended.
Type of specimen
Clinical samples - rectal screening swabs, faecal samples or from isolated colony.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,3).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Slight colour variation may be observed depending upon strains.
- Isolated colonies should not be directly plated on to this medium, because the high level inoculum may cause false positive results.
- Further confirmation using biochemical identification tests is recommended.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile HiCrome™ ESBL Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles.
Colour of medium: Yellow coloured medium
Quantity of medium: 25ml of medium in 90mm plate
pH: 6.60-7.00
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Colony |
|---|---|---|---|
| Escherichia coli NCTC 13351 | 50-100 | luxuriant | pink to purple |
| $ Klebsiella quasipneumoniae ATCC 700603 | 50-100 | luxuriant | bluish green |
| Enterobacter cloacae ATCC 23355 | >=103 | inhibited | - |
| Citrobacter freundii ATCC 8090 | >=103 | inhibited | - |
| Candida albicans ATCC 10231 (00054*) | >=103 | inhibited | - |
Key: (*) Corresponding WDCM numbers, ($)Formerly known as Klebsiella pneumoniae.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,3).
| Product Name | HiCrome™ ESBL Agar Plate |
|---|---|
| SKU | MP1829 |
| Customized Product Available | No |
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