Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for determining efficiency of containers, equipment surfaces, water miscible cosmetics and inactivation of ẞ lactam antibiotics.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 15.000 |
| Soya Peptone | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Agar | 15.000 |
| ẞ-lactamase I | 330.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Tryptone Soya Agar with Lecithin and Polysorbate 80 is used in RODAC (Replicate Organism Detection and Counting) plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2,3). Tryptone and Soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 (Tween 80) are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5).
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (6). After counting the colonies, carry out biochemical testing for identification.
Type of specimen
Swabs of containers, Equipment surfaces, Water miscible cosmetics etc.
Specimen Collection and Handling
For swabs of containers, equipment surfaces, water miscible cosmetics samples follow appropriate techniques for handling specimens as per established guidelines (1).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets..
Limitations
- 1.Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- 2.Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- 3.It is recommended to store the plates at 24-30°C to avoid minimum condensation.
- 4. Further biochemical test must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Tryptone Soya Agar w/ Lecithin and Tween 80, w/ ẞ-lactamase in 90mm disposable plate with smooth surface and absence of black particles/cracks/bubbles
Colour
Light yellow coloured medium.
Quantity of Medium
25ml of medium in 90 mm disposable plates.
Sterility check
Passes release criteria
Reaction
7.10-7.50
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was o bserved after incubation at 30-35°C for < = 3 days. Simultaneously growth promotion test was carried out on plates which were seeded with 100 mcg/0.1ml of Benzyl Penicillin.
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Growth w/ antibiotic | Recovery w/ antibiotic |
|---|---|---|---|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
| Proteus mirabilis ATCC 25933 | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | 35-100 | >=70% | luxuriant | >=70% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 20-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Soyabean Casein Digest Agar Plate w/ Lecithin and Tween 80, w/ β-Lactamase (Tryptone Soya Agar Plate w/ Lecithin and Tween 80 w/ β-Lactamase) |
|---|---|
| SKU | MP1809 |
| Customized Product Available | No |
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