Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Soyabean Casein Digest Agar Plate w/ Lecithin and Tween 80, w/ β-Lactamase II (Tryptone Soya Agar Plate w/ Lecithin and Tween 80 w/ β-Lactamase II) (γ-irradiated) (Triple Pack)
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Recommended Use
Recommended for determining efficiency of containers, equipment surfaces, water miscible cosmetics and for inactivation of cephalosporins of first, second, third and fourth generation.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 15.000 |
| Papaic digest of soyabean meal | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Agar | 15.000 |
| Glycerol | 1.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate. Alternatively this medium can also be used for environmental monitoring of clean rooms in production areas of Pharmaceutical industries where production of antibiotics like Penicillins and Cephalosporins is carried out.
Principle And Interpretation
Soyabean Casein Digest Agar with Lecithin and Polysorbate 80 and beta-lactamase is used in plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2, 3) and also in environmental monitoring of clean room for facilities where production of Penicillins and Cephalosporins is carried out.
Casein enzymic hydrolysate and papaic digest of soyabean meal provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 (Tween 80) are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5).
Beta-lactamase added in the medium will inactivate the beta-lactam antibiotics thus enabling the growth of resistant strains present in the environment of clean rooms where production of antibiotics is carried out.
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar w/Tween 80 and lecithin and W/ 4.5 IU per plate of beta-Lactamase II in 90mm plate (gamma-irradiated) (Triple pack).
Colour
Light yellow coloured medium.
Quantity of Medium
30ml of medium in 90mm plates.
Reaction
7.10-7.50
Dose of Irradiation (Kgy)
15.00-25.00
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was observed after incubation at 30-35°C for <= 3 days. Simultaneously growth promotion test was carried out on plates which were seeded with 1 mcg/ml of respective antibiotics.
Recovery Rate
Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar.
Sterility Test
Passes release criteria.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| w/ Cephalothin | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefamandole | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefotaxime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Ceftazidime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefepime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Imipenem | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| Staphylococcus aureus ATCC 25923 | ||||||
| w/o antibiotic | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cephalothin | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefamandole | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefotaxime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Ceftazidime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Cefepime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/ Penicillin | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| Staphylococcus aureus ATCC 29213 | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| w/ Penicillin | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| Pseudomonas aeruginosa ATCC 27853 | ||||||
| w/o Antibiotic | 50-100 | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| w/Cefotaxime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/Ceftazidime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/Cefepime | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| w/Imipenem | Luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs | |
| Growth promoting | ||||||
| Candida albicans ATCC 10231 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | Good-luxuriant | 25 -70 | 50-70% | 30-35 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | Luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
| Product Name | Soyabean Casein Digest Agar Plate w/ Lecithin and Tween 80, w/ β-Lactamase II (Tryptone Soya Agar Plate w/ Lecithin and Tween 80 w/ β-Lactamase II) (γ-irradiated) (Triple Pack) |
|---|---|
| SKU | MP1808GT |
| Customized Product Available | No |
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