Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for determining efficiency of containers, equipment surfaces, water miscible cosmetics and for inactivation of penicillins, cephalosporins of first, second, third and fourth generation and penems.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 15.000 |
| Soya Peptone | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Tween 80 (Polysorbate 80) | 5.000 |
| β-Lactamase mixture | 500 IU |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.30±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Tryptone Soya Agar with Lecithin and Tween 80 is used for the detection and enumeration of microorganisms present on surfaces of sanitary importances (1,2,3). Tryptone and Soya peptone provide nitrogenous, carbonaceous compounds, long chain amino acids and other nutrients essential for microbial replication. Lecithin and Tween 80 are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and Tween 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5). β-Lactamase enzyme breaks the ẞ-Lactam ring of antibiotic, deactivating the molecule's antibacterial properties (6,7). Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (8). After counting the colonies, carry out biochemical testing for identification.
Type of specimen
Products of sanitary importance, water miscible cosmetics
Specimen Collection and Handling
Follow appropriate techniques for handling specimens as per established guidelines (5).
Swab specimens can be directly streaked on the plate. Liquid specimens can be inoculated by means of an inoculation loop.
Warning and Precautions
Read the label before opening the plate. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical testing is required for complete identification.
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay in development.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Soyabean Casein Digest Agarw/ Lecithin and Tween 80 w/ ẞ-Lactamase mixture in 90mm plate with smooth surface and absence of black particles/ cracks/ bubbles
Colour of medium: Light yellow coloured medium.
Quantity of Medium: 30ml of medium in 90mm plates.
pH: 7.10-7.50
Sterility Check: Passes release criteria
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was observed after incubation at 30-35° C for <= 3 days. Simultaneously growth promotion test was carried out on plates which were seeded with 500 mcg/ml of respective antibiotics.
Recovery Rate: Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar
| Organism | Inoculum (CFU) | Growth | Lot value (CFU) | Incubation temperature | Incubation period | Recovery |
|---|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% |
| w/ Cephalothin | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefamandole | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefotaxime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Ceftazidime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefepime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Imipenem | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| Staphylococcus aureus subsp aureus ATCC 25923 (00034*) | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% |
| w/ Cephalothin | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefamandole | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefotaxime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Ceftazidime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Cefepime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/ Penicillin | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| Staphylococcus aureus ATCC 29213 (00131*) | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% |
| w/ Penicillin | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
Growth promoting
| Organism | Inoculum (CFU) | Growth | Lot value | Incubation temperature | Incubation period | Recovery |
|---|---|---|---|---|---|---|
| Pseudomonas aeruginosa ATCC 27853 (000024*) | ||||||
| w/o antibiotic | 50-100 | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% |
| w/Cefotaxime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/Ceftazidime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/Cefepime | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| w/Imipenem | Luxuriant | 35-100 | 30-35 °C | 18-24 hrs | >=70% | |
| Growth promoting | ||||||
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | 35-100 | 30-35 °C | <=5 d | >=70% |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 35-100 | 30-35 °C | <=5 d | >=70% |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | 35-100 | 30-35 °C | <=5 d | >=70% |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | Luxuriant | 35-100 | 20-25 °C | <=5 d | >=70% |
Key: (#) Formerly known as Aspergillus niger, (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Soyabean Casein Digest Agar Plate w/ Lecithin and Tween 80 w/ β-Lactamase mixture (Tryptone Soya Agar Plate w/ Lecithin and Tween 80 w/ β-Lactamase mixture) |
|---|---|
| SKU | MP1807 |
| Customized Product Available | No |
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