Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended as a general purpose medium for cultivation of wide variety of organisms and for inactivation of ẞ-lactam antibiotics.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| ẞ Lactamase mixture | 330 IU/lit |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Pancreatic digest of casein
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Soyabean Casein Digest Agar is recommended by various pharmacopoeias and is in accordance with the microbial limit testing harmonizedmethodology of USP/EP/BP/JP/IP (1-5).Tryptone Soya Agar with ẞ Lactamase mixture is used in plates for the detection and enumeration of microorganisms present on surfaces of sanitary importances and also in environmental monitoring of clean room for facilities where production of Cephalosporins is carried out.
Tryptone and soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. ẞ Lactamase inactivates ẞ-lactam antibiotics. thus enabling the growth of resistant strains present in the environment of clean rooms where production of antibiotics is carried out.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling:
For Pharmaceutical samples follow appropriate techniques for sample collection, handling and processing as per pharmacopoeias (1-5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- It is recommended to store the plates at 24-30°C to avoid minimum condensation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar Plate w/ ẞ Lactamase in 90mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles
Colour
Light yellow coloured medium
Quantity of Medium
30ml of medium in 90 mm disposable plate.
Sterility Check
Passes release criteria
pH
7.10-7.50
Concentration
Concentration of Penicillinase added: 330 IU/lit of media (approximately 2,00,000 LU/lit)
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was observed after incubation at 30-35°C for <= 3 days.
Simultaneously growth promotion test was carried out on plates which were seeded with 100 mcg/0.1ml of Benzyl Penicillin.
Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar.
| Organism | Inoculum (CFU) | Growth | Recovery | Growth w/ antibiotic | Recovery w/ antibiotic |
|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 29213 (00131*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
Key: * - Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 20-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Soyabean Casein Digest Agar Plate w/ β Lactamase (Tryptone Soya Agar Plate w/ β Lactamase) |
|---|---|
| SKU | MP1765 |
| Customized Product Available | No |
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