Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
for the cultivation of yeasts, moulds and aciduric bacteria and can be used for determining efficiency of sanitization of containers, equipment surfaces, water wmiscible cosmetics etc.
Composition
| Ingredients | g / L |
|---|---|
| Dextrose (Glucose) | 40.000 |
| Mycological peptone | 10.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Sabouraud Dextrose Agar with Soya Lecithin and Polysorbate 80 is the modification of formulation described by Sabouraud for determining efficiency of sterilization of container etc with respect to yeast moulds and aciduric bacteria(1).
Mycological peptone provides nitrogenous compounds. Dextrose provides an energy source. The low pH favours fungal growth and inhibits contaminating bacteria from specimens (2). Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Lecithin and polysorbate 80 are neutralizers reported to inactivate residual disinfectants from where the sample is collected (3). Lecithin neutralizes quartenery ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene formalin, and with lecithin neutralizes ethanol (4). Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of fungi is determined by the appearance of colonies on the agar surface.
Type of specimen
Environmental samples
Specimen Collection and Handling
For samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- It is recommended to store the plates at 24-30°C to avoid minimum condensation.
- Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Sabouraud Dextrose Agar w/ Lecithin & Polysorbate 80 in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles (y irradiated, Triple pack)
Colour of the medium: Light amber coloured medium.
Quantity of Medium: 30ml of medium in 90mm disposable plates
pH: 5.40-5.80
Dose of Irradiation (Kgy): 13.00-20.00
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
| Organism | Inoculum | Growth | Recovery |
|---|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | >=50% |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=50% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant | >=50% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | >=50% |
| Trichophyton rubrum ATCC 28191 | 50-100 | luxuriant | >=50% |
Key: (*) - Corresponding WDCM numbers.
# - Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 20-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Sabouraud Dextrose Agar Plate w/Lecithin & Polysorbate 80 (γ-irradiated) (Triple pack) |
|---|---|
| SKU | MP1659GT |
| Customized Product Available | No |
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