Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended a selective medium used to detect and isolate Salmonella and Shigella species. Also useful for isolation of Vibrio cholerae.
Composition
| Ingredients | g/L |
|---|---|
| Proteose peptone | 7.000 |
| HM peptone B # | 3.000 |
| Lactose | 5.000 |
| Saccharose (Sucrose) | 5.000 |
| Sodium citrate | 10.000 |
| Sodium thiosulphate | 5.000 |
| Sodium deoxycholate | 2.500 |
| Neutral red | 0.030 |
| Agar | 12.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Meat extract B
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Salmonella infection leads to salmonellosis, which ranges clinically from self-limited gastroenteritis (diarrhea, abdominal cramps and fever) to enteric fevers (including typhoid fever). Shigella species cause classical bacillary dysentery characterized by severe cramping abdominal pain and diarrhea with blood and mucus. D.C.L.S. Agar is a modification of Deoxycholate Citrate Agar of Leifson (1). It is a slightly selective and differential medium, which incorporates sucrose as an additional fermentable carbohydrate to differentiate lactose negative sucrose positive coliforms from Salmonella species. The addition of sucrose to this medium increases its usefulness because non-pathogenic sucrose fermenting organisms like Proteus, Enterobacter, Klebsiella form red colonies. D.C.L.S. Agar is a moderately selective culture medium which also supports the growth of Vibrio species.
Type of specimen
Clinical samples - Stool samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile D.C.L.S. Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Reddish orange coloured medium.
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis ATCC>=104 29212(*00087) | inhibited | 0% | ||
| Escherichia coli ATCC 25922(00013*) | 50-100 | none-poor | <=10% | red |
| ## Proteus hauseri ATCC 13315 | 50-100 | luxuriant | >=50% | red |
| Salmonella Typhimurium ATCC 14028(00031*) | 50-100 | luxuriant | >=50% | colourless- slightly pink |
| Shigella flexneri ATCC 12022(00126*) | 50-100 | fair-good | 30-40% | colourless- slightly pink |
Key: (*) Corresponding WDCM numbers.
(##)Formerly known as Proteus vulgaris
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | D.C.L.S. Agar |
|---|---|
| SKU | MP160 |
| Customized Product Available | No |
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