Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the selective isolation and cultivation of Corynebacterium species.
Composition
| Ingredients | g / L |
|---|---|
| Biopeptone | 10.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 4.000 |
| Corn starch | 1.000 |
| Potassium dihydrogen phosphate | 1.000 |
| Agar | 10.000 |
| Haemoglobin solution(FD022) | 1 vial |
| Vitamino Growth Supplement (FD025) | 1 vial |
| 1%Potassium Tellurite (FD052) | 1 vial |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Corynebacterium is a genus of gram-positive, facultatively anaerobic, non-motile bacteria that exhibits a fermentative metabolism (carbohydrates to lactic acid) under certain conditions. Corynebacteria constitute a diverse group of bacteria that includes saprophytic associations as well as plant and animal pathogens. Most species are normal flora of humans present virtually at all anatomic sites. Many species of Corynebacteria can be isolated from various places such as soil, water, blood, and human skin. Pathogenic strains of Corynebacteria can infect plants, animals, or humans. Tellurite Blood Agar is a selective medium used for isolation and cultivation of Corynebacterium species (1,2).
It is selective due to the presence of inhibitor and differential by means of ability of organism to reduce potassium tellurite. Biopeptone provides nitrogenous compounds. Sodium chloride maintains the osmotic equilibrium of the medium while phosphates buffer the medium. Corn starch neutralizes the toxic metabolites. Haemoglobin and Vitamino Growth Supplement stimulate good growth of Corynebacterium. Potassium tellurite acts as a selective agent and has inhibitory activity against most gram-positive and gram-negative bacteria except Corynebacterium species. C.diphtheriae reduces potassium tellurite to tellurium and thereby produce gray-black coloured colonies. Throat or nasal swab is directly inoculated and streaked on this agar medium.
Type of specimen
Clinical samples - throat swab
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the pack. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Specimens if enriched on Loeffler medium, can give better growth of Corynebacterium species.
- Other organisms such as Staphylococci, Streptococci will grow as minute black colonies due to tellurite reduction, hence Corynebacterium should be confirmed by gram staining and other biochemical test.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Tellurite Blood Agar Base in 90 mm disposable plates with smooth surface and absence of black particles/cracks/ bubbles
Colour of medium: Reddish brown coloured
Quantity of medium: 25 ml of medium in 90 mm disposable plates.
Reaction: 7.00-7.40
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 48 hours (or more).
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Corynebacterium diphtheriae ATCC 11913 | 50-100 | good-luxuriant | >=50% | grey-black |
| Escherichia coli ATCC 25922 (00013*) | >=103 | inhibited | 0% | - |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Tellurite Blood Agar Plate |
|---|---|
| SKU | MP1260 |
| Customized Product Available | No |
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