Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For detection of pyocyanin production by Pseudomonas species.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Potassium sulphate | 10.000 |
| Magnesium chloride | 1.400 |
| Glycerol | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Pseudomonas Agar is based on the formulation described by King et al (1) and as recommended in U.S. Pharmacopoeia (2) for detecting pyocyanin, a water soluble pigment by Pseudomonas species (3). This medium enhances the elaboration of pyocyanin but inhibits the formation of fluorescein pigment. The fluorescein pigment diffuses from the colonies of Pseudomonas into the agar and shows blue colouration. Some Pseudomonas strains produce small amounts of fluoresce in resulting in a blue-green colouration.
Peptone supply carbon, nitrogen substances, amino acids, other essential growth nutrients. Potassium sulphate and magnesium chloride, which enhances the pyocyanin production and suppresses the fluorescein production. A pyocyanin-producing Pseudomonas strain will usually also produce fluorescein. It must, therefore, be differentiated from other simple fluorescent Pseudomonads by other means. Temperature can be a determining factor as most other fluorescent strains will not grow at 35°C. Rather, they grow at 25-30°C (3).
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological test are to be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Pseudomonas Pyocyanin Agar Plate in 90 mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles
Colour of medium: Yellow coloured medium
Quantity of medium: 25 ml of medium in 90 mm disposable plates.
pH: 6.80-7.20
Sterility Check: Passes release criteria
Cultural Response
Cultural response was observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Colour of Medium |
|---|---|---|---|---|---|
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant | 25-100 | >=50% | blue-green |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | 25-100 | >=50% | blue-green |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Pseudomonas Pyocyanin Agar Plate |
|---|---|
| SKU | MP119 |
| Customized Product Available | No |
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