Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the cultivation and maintenance of recombinant strains of E.coli for genetic and molecular biology studies
Composition
| Ingredients | Gms/Litre |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Kanamycin | 0.1 |
| Final pH (at 25°C) | 7.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Luria Bertani Agar is prepared as described by Lennox (4) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (1) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Tryptone provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Type of specimen
Recombinant strains of E.coli
Specimen Collection and Handling
For Recombinant strain samples follow appropriate techniques for handling specimens as per established guidelines (1,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Luria Bertani Agar Plate w/100µg/ml Kanamycin in 90mm disposable plates.
Colour of medium: Yellow to amber coloured medium
Quantity of medium: 25ml of medium in disposable plate
Reaction: 7.30-7.70
Sterility test: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Growth |
|---|---|
| Escherichia coli DH5 alpha (MTCC 1652) | Inhibited |
| Escherichia coli BL21 (MTCC 1679) | Inhibited |
| Escherichia coli DH5 alpha (MTCC 1652) Transformed strain | luxuriant |
| Escherichia coli BL21 (MTCC 1679)Transformed strain | luxuriant |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Luria Bertani Agar Plate w/100µg/ml Kanamycin |
|---|---|
| SKU | MP1151K |
| Customized Product Available | No |
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