Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for differential and selective isolation of Salmonella and Shigella species from pathological specimens, suspected foodstuffs etc.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| HM peptone B # | 5.000 |
| Lactose | 10.000 |
| Bile salts mixture | 8.500 |
| Sodium citrate | 10.000 |
| Sodium thiosulphate | 8.500 |
| Ferric citrate | 1.000 |
| Brilliant green | 0.00033 |
| Neutral red | 0.025 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
SS Agar medium is recommended as differential and selective medium for the isolation of Salmonella and Shigella species from pathological specimens (1) and suspected foodstuffs (2,3,4,5) and for microbial limit test (6). SS Agar is a moderately selective medium in which gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate. Peptone, HM peptone B provides nitrogen and carbon source, long chain amino acids, vitamins and essential growth nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate selectively inhibit gram-positive and coliform organisms. Sodium thiosulphate is reduced by certain species of enteric organisms to sulphite and H2S gas and this reductive enzyme process is attributed by thiosulphate reductase. Production of H2S gas is detected as an insoluble black precipitate of ferrous sulphide, formed upon reaction of H2S with ferric ions or ferric citrate, indicated in the center of the colonies.
The high selectivity of Salmonella Shigella Agar allows the use of large inocula directly from faeces, rectal swabs or other materials suspected of containing pathogenic enteric bacilli. On fermentation of lactose by few lactose-fermenting normal intestinal flora, acid is produced which is indicated by change of colour from yellow to red by the pH indicator-neutral red. Thus these organisms grow as red pigmented colonies. Lactose non-fermenting organisms grow as translucent colourless colonies with or without black centers. Growth of Salmonella species appears as colourless colonies with black centers resulting from H2S production. Shigella species also grow as colourless colonies which do not produce H2S.
Type of specimen
Clinical: faeces, rectal swabs, etc.; Suspected food stuffs.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,3,4). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The medium is highly selective and may be toxic to certain Salmonella or Shigella species. Hence it is recommended to use to inoculate plates of less inhibitory media parallel to SS Agar, such as Hektoen Enteric Agar (M467) or Deoxycholate Citrate Agar (M065) for easier isolation of Shigella species (3).
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile SS Agar (Salmonella Shigella Agar) in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Reddish orange coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
6.80-7.20
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | fair | 20-30% | cream pink |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | fair | 20-30% | pink with bile precipitate |
| Salmonella Choleraesuis ATCC 12011 | 50-100 | good-luxuriant | >=50% | colourless with black centre |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | colourless with black centre |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | none-poor | <=10% | colourless |
| Proteus mirabilis ATCC 25933 | 50-100 | fair-good | 30-40% | colourless, may have black centre |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | good | 40-50% | colourless |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=50% | colourless with black centre |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | colourless with black centre |
Key: (*) Corresponding WDCM numbers. (#)Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | SS Agar (Salmonella Shigella Agar) Plate |
|---|---|
| SKU | MP108 |
| Customized Product Available | No |
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