Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for cultivation of yeasts, moulds and aciduric microorganisms.
Composition**
| Ingredients | g/L |
|---|---|
| Dextrose (Glucose) | 40.000 |
| Mycological, peptone | 10.000 |
| Glycerol | 10.000 ml |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Sabouraud Dextrose Agar is Carlier's modification (1) of the formulation described by is a modification of Sabouraud Dextrose Agar which is described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds). This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3). Mycological Peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from test samples (4).
Type of specimen
Food & Dairy samples; Cosmetics;
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines(7,8,9). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
For professional use only. Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1.Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- 2.Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- 3.Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet
- 4. Further biochemical tests should be carried out for confirmation.
- 5.It is recommended to store the plates at 24-30°C to avoid minimum condensation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Sabouraud Dextrose Agar Plate w/1% Glycerol in 90 mm disposable plates with smooth surface and absence of black particles/ bubbles and cracks.
Colour of medium
Light amber coloured medium
Quantity of medium
32 ml of medium in 90 mm disposable plates.
pH
5.40-5.80
Sterility Check
Passes release criteria
Dose of irradiation (Kgy)
13.00-20.00
Cultural Response
Growth Promotion was carried out in accordance with the (USP/EP/BP/JP), after an incubation at 20-25°C for 24-48 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | Luxuriant (white colonies) | >=70% |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| $Lactobacillus paracasei ATCC 334 | 50-100 | luxuriant | >=70% |
| Trichophyton rubrum ATCC 28191 | luxuriant |
Key: *Corresponding WDCM numbers. $Formerly known as Lactobacillus casei. # Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Sabouraud Dextrose Agar Plate w/ 1% Glycerol (γ-irradiated) (Triple Pack) |
|---|---|
| SKU | MP063AGT |
| Customized Product Available | No |
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