Bacterial Transformation Kit

Bacterial transformation is a process which involves genetic alteration of bacteria by incorporation and stable expression of a foreign genetic material from the environment or surrounding medium. For the incorporation of plasmid into a cell, bacteria must first be made “competent”. This process includes the treatment of cells with bivalent metal ions in ice-cold condition. As a result small pores are formed onthe cell membrane, which makes it permeable. The plasmid DNA may adhere to the surface of the celland uptake is mediated by a pulsed heat shock. HiMedia’s BacterialTransformation Kit provides a very simple and convenient method for preparation of chemically competent E.coli cells which are commonly used in cloning experiments. This kit contains two Transformation Buffers, I and II, for preparation of competent cells in a few steps.