Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium I
Used for the microbiological assay of Bleomycin using Mycobacterium smegmatis, in accordance with Indian Pharmacopoeia.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 10.000 |
| Beef extract | 10.000 |
| Sodium chloride | 3.000 |
| Agar | 17.000 |
| pH after sterilization | 7.0±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml purified/distilled water containing 10 grams glycerine. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Bleomycin
Principle And Interpretation
This medium is formulated in accordance with IP and CFR (1,2).This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis. The nutrients essential for growth of test organism is provided by peptone and beef extract in this medium. Agar provides excellent solid substratum for support and overlayering of seed agar, for the assay of Bleomycin. Addition of glycerine is important for provision of carbon to the test organism.
To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 1.7% agar gel.
Colour and Clarity of prepared medium: Medium amber coloured clear to slightly opalescent gel forms in Petri plates.
pH: pH of 4.0% w/v aqueous solution containing 1% glycerine after sterilization. pH: 7.0±0.1
Cultural Response
MM798: Cultural characteristics observed after an incubation at 36-37.5°C for 48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Mycobacterium smegmatis ATCC 607 | 50-100 | luxuriant | >=70% | Bleomycin |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium I |
|---|---|
| SKU | MM798 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983Title 21, Part 436, Subpart D,Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April 1). |
| Customized Product Available | No |
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