Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
This medium is recommended for the selective subculture of Salmonella species in accordance with Indian Pharmacopoeia 2014.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Part A | |
| Peptone | 10.000 |
| Meat extract B # | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.4±0.2 |
| Solution (i) Bismuth Sulphite Glucose Phosphate mixture (Part B) | (Gms/200ml) |
| Bismuth ammonio-citrate scales | 6.000 |
| Sodium sulphite | 20.000 |
| Disodium hydrogen phosphate | 20.000 |
| Glucose | 10.000 |
| Solution (ii) Iron citrate brilliant green mixture (Part C) | (Gms/45ml) |
| Ferric citrate, brown scales | 0.400 |
| Brilliant green | 0.050 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 4.5 grams of Part A in 100 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Suspend 5.6 grams of Part B [Bismuth Sulphite Glucose Phosphate Mixture, solution (i)] in 20 ml sterile purified/ distilled water, boil the solution till all the ingredients are properly dissolved. Suspend 0.045 grams of Part C [Iron Citrate Brilliant Green Mixture, solution (ii)] in 4.5 ml sterile purified/ distilled water. Mix aseptically 20 ml of Part B and 4.5 ml of Part C with 100 ml of previously melted sterile Nutrient Agar (Part A) and cool to a temperature 60°C and pour into sterile Petri plates.
Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this. User must ensure its compatibility with the latest edition.
Principle And Interpretation
Salmonella is a genus of gram-negative Enterobacteriaceae - commonly implicated in foodborne illness and the causative agent of typhoid and paratyphoid fever. Salmonella species have been isolated from humans and animals. More than 2000 serovars of Salmonella exists with each showing different host specificities. For example, humans are the only known natural reservoir for serotype Salmonella Typhi and serotypes Salmonella Paratyphi A, B and C (1). The organism can be transmitted by the faecal-oral route. It is excreted by humans in faeces and may be transmitted by contaminated water, food, or by person-to-person contact (with inadequate attention to personal hygiene).
Wilson and Blair's BBS Agar formulated by Wilson and Blair (2) is recommended for isolating Salmonella species especially Salmonella Typhi from clinical specimens. The selective reagent formulation is a modification of the bismuth sulphite reagent described by Hajna and Perry (3). This medium is particularly valuable for the isolation of S.Typhi . The medium is highly selective for Salmonellae, being inhibitory to coliforms, Proteus; occasional strains of coliforms grow to form dull green or brown colonies, but without a surrounding metallic sheen. This medium is recommended by Indian Pharmacopoeia for the selective subculture of Salmonella after enrichment in Rappaport Vassiliadis Salmonella Enrichment Broth (5).
medium is also suitable for the isolation of lactose-fermenting strains of Salmonellae (which can not be differentiated on lactose containing differential media) since lactose is not the fermentable substrate used in this medium (4).
Peptone and meat extract B provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other growth nutrients. Sodium chloride maintains the osmotic balance. Brilliant green dye inhibits all gram positive bacteria. Glucose is the fermentable carbohydrate. Bismuthis a heavy metal, which is inhibitory to most gram-negative enteric bacilli other than Salmonella. Ferric citrate is reduced by Salmonella species in presence of bismuth amonium citrate and glucose to form iron sulphide, indicated by black coloured colonies. Disodium hydrogen phosphate buffers the medium well.
Quality Control
Appearance
Part A: Cream to yellow homogeneous free flowing powder Part B: White to offwhite homogeneous free flowing powder Part C: Green homogeneous free flowing granules
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
After addition of Part B and Part C to Part A:Greenish yellow coloured, opaque gel forms in Petri plates.
Cultural Response
Growth Promotion is carried out in accordance with the method of IP. Cultural response was observed after an incubation at 30-35°C for 24-48 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35°C for <=24 hours).
Indicative properties
Colonies are comparable in appearance and indication reaction to those previously obtained with previously tested and approved lot of medium occurs for the specified temperature for a period of time within the range specified inoculating <=100cfu (at 30-35°C for 24-48 hours).
Inhibitory properties
No growth of the test microorganism occurs at the specified temp for not less than longest period of time specified inoculating >=100cfu (at 30-35°C for >= 48 hours).
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Colour of Colony | Incubation temperature |
|---|---|---|---|---|---|---|
| Growth Promoting + Indicative | ||||||
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 25-100 | >=50% | Green colonies with black centre to black with metallic sheen | 24-48 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | good-luxuriant | 25-100 | >=50% | Green colonies with black centre to black with metallic sheen | 24-48 hrs |
| Inhibitory properties | ||||||
| Escherichia coli ATCC 8739 | >=103 | Inhibited | 0 | 0% | >=48 hours | |
| Shigella boydii ATCC 8700 | >=103 | Inhibited | 0 | 0% | >=48 hours | |
| Additional Microbiological Testing | ||||||
| Proteus mirabilis ATCC 25933 | 50-100 | luxuriant | 25-100 | >=50% | green | 24-48 hrs |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant | 25-100 | >=50% | black with sheen | 24-48 hrs |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | 25-100 | >=50% | black with sheen | 24-48 hrs |
| Escherichia coli ATCC 25922 | >=103 | Inhibited | 0 | 0% | >=48 hrs | |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry period on the label.
| Product Name | Wilson and Blair's BBS Agar (Medium 10) (Triple pack) |
|---|---|
| SKU | MM331 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Murray P.R., Baron J.H., Pfaller M.A., Jorgensen J.H. and Yolken R.H.,(Ed.), 2003, Manual of Clinical Microbiology, 8thEd.,American Society for Microbiology, Washington, D.C. 2.Wilson W. J. and Blair E. M., 1926, J. Pathol. Bacteriol., 29 : 310. 3.Hajna A. A. and Perry C. A., 1938, J. Lab. Clin. Med., 23:1185. 4.Collee J.g., Fraser A.g., Marmion B.P., Simmons A., (Eds), Mackie and MC Cartney, Practical Medical Microbiology, 1996,14th Edition, Churchill Livingstone5.The Indian Pharmacopoeia 2014,Goverment of India 2014, The Controller of Publication. |
| Customized Product Available | No |
Write Your Own Review
Wilson Blair HiVeg™ Agar Base
As low as
₹1,788.00
Wilson Blair Agar Base
As low as
₹1,693.00
Upsell Products
HiCrome™ RajHans Medium (Salmonella Agar)
As low as
₹6,478.00
HiCrome™ Salmoconfirm Selective Agar
As low as
₹13,405.00
Brilliant Green Agar, Modified
As low as
₹2,478.00
Brilliant Green Agar Modified Plate
As low as
₹1,044.00
Xylose Lysine Deoxycholate Agar (XLD Agar) Plate
As low as
₹2,000.00
XLD Agar Plate, Modified
As low as
₹2,000.00
SS Agar (Salmonella Shigella Agar) Plate
As low as
₹2,000.00
HiCrome™ Salmonella Agar Plate
As low as
₹2,230.00