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Sabouraud Dextrose Agar Medium w/ Tetracycline (In a accordance with IP 2010)
For selective cultivation of yeasts and moulds in accordance with Indian Pharmacopoeia.
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Intended Use
Sabouraud Dextrose Agar Medium with antibiotics is recommended for selective cultivation of yeasts and moulds in accordance with Indian Pharmacopoeia, 2010.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptones (meat and casein) | 10.000 |
| Dextrose monohydrate | 40.000 |
| Agar | 15.000 |
| pH after sterilization (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 61.36 grams (the equivalent weight of dehydrated medium) in 995 ml purified/distilled water. Heat to boiling to dissolve the mediumcompletely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Aseptically add rehydrated contents of one vial of Tetracycline Selective Supplement (FD196). Mix well before pouring into sterile Petri plates.
Principle And Interpretation
Sabouraud Dextrose Agar Medium w/ antibiotics is recommended for cultivation of yeasts and moulds by Indian Pharmacopoeia (1). This medium was described originally by Sabouraud (2) for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is used with antibiotics such as tetracycline and benzylpenicillin (3) for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria. Peptic digest of animal tissue and pancreatic digest of casein provide nitrogenous compounds. Dextrose provides an energy source. Tetracycline and benzyl penicillin inhibits a wide range of Gram-positive and Gram-negative bacteria which makes the medium selective for fungi (4). The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (5).
Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
pH: 5.40-5.80
Growth Promotion Test: Cultural response was carried out in accordance with IP, with added Tetracycline Selective Supplement (FD196), after an incubation at 20-25°C for <=5 days. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Candida albicans ATCC 10231 | 50-100 | Luxuriant (white colonies) | 25-100 | >=50% | 20-25 °C | <=5 d |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | 25-100 | >=50% | 20-25 °C | <=5 d |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 25-100 | >=50% | 20-25 °C | <=5 d |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | 35-100 | >=50% | 20-25 °C | <=5 d |
| Escherichia coli ATCC 25922 | >=10³ | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Escherichia coli ATCC 8739 | >=10³ | inhibited | 0 | 0% | 20-25 °C | <=5 d |
| Escherichia coli NCTC 9002 | >=10³ | inhibited | 0 | 0% | 20 -25 °C | <=5 d |
| Trichophyton rubrum ATCC 28191 | 50-100 | good | 20-25 °C | <=7 d | ||
| Lactobacillus casei ATCC 334 | >=10³ | inhibited | 0 | 0% | 20-25 °C | <=5 d |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Sabouraud Dextrose Agar Medium w/ Tetracycline (In a accordance with IP 2010) |
|---|---|
| SKU | MM1472 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Indian Pharmacopoeia 2010, Ministry of Health and Family welfare, Government of India, New Delhi 2.Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061. 3.Ajello L., 1957, J. Chron. Dis., 5:54 |
| Customized Product Available | No |
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