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Fluid Thioglycollate Medium
For sterility testing of biologicals and For cultivation of aerobes, anaerobes and microaerophiles in accordance with Indian Pharmacopoeia.
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Intended Use
Recommended for sterility testing of biologicals and for cultivation of aerobes, anaerobes and microaerophiles in accordance with IP.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Tryptone # | 15.000 |
| Yeast extract | 5.000 |
| Dextrose monohydrate | 5.500 |
| Sodium chloride | 2.500 |
| L-Cystine | 0.500 |
| Sodium thioglycollate | 0.500 |
| Resazurin sodium | 0.001 |
| Agar | 0.750 |
| pH after sterilization | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Pancreatic digest of casein
Directions
Suspend 29.25 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. Cool to 25°C and store in a cool dark place preferably below 25°C.
Note: If more than the upper one-third of the medium has acquired a pink-purple colour, the medium may be restored once by heating in a water bath or in free flowing steam until the pink-purple colour disappears.
Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this. User must ensure its compatibility with the latest edition.
Principle And Interpretation
Brewer (1) formulated Fluid Thioglycollate Medium for rapid cultivation of aerobes as well as anaerobes by adding a reducing agent and small amount of agar. The Indian Pharmacopoeia (2) and AOAC (9) have recommended the media for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. However, it is intended for the examination of clear liquid or water-soluble materials.
Tryptone and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other growth factors necessary for bacterial multiplication. Dextrose monohydrate is carbohydrate source. Sodium thioglycollate and L-cystine act as a reducing agent lowering the oxidation-reduction potential by removal of oxygen This condition helps to prevent the accumulation of peroxides which is toxic in nature. The SH group also neutralizes the antibacterial effect of mercurial preservatives and other heavy metal compounds which exert a bacteriostatic effect in the materials under examination. Any increase in the oxygen content is indicated by a colour change of redox indicator, resazurin to red (6,7,8). The small amount of agar helps in maintaining low redox potential for stabilizing the medium (5).
In sterility checking, it is recommended to dilute sample containing preservatives, using this broth to reduce the toxicity and enhance the growth of contaminants, if any.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines (2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- It is intended for the examination of clear liquid or water-soluble materials.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light straw coloured, clear to slightly opalescent solution with upper 10% or less medium pink - purple on standing.
Reaction
Reaction of 2.97% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Growth Promotion Test
As per Indian Pharmacopoeia
Stability test
Light yellow coloured clear solution without any precipitation sedimentation at room temperature for 7 days
Cultural response
Cultural characteristics observed after an incubation at 30-35°C for not more than 3 days.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Clostridium sporogenes ATCC 19404 (00008*) | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
| Clostridium sporogenes NBRC 14293 | 50-100 | luxuriant |
| Clostridium perfringens ATCC 13124 (00007*) | 50-100 | luxuriant |
| Bacteroides fragilis ATCC 23745 | 50-100 | luxuriant |
| Bacteroides vulgatus ATCC 8482 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Staphylococcus aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
Key: * Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Fluid Thioglycollate Medium |
|---|---|
| SKU | MM009 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Brewer, 1940, J. Am. Med. Assoc., 115:598. 2.Indian Pharmocoepoeia, 2010 , Ministry of Health and Family Welfare, Govt. of India. 3.Williams. (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th ed., AOAC,Washington, D.C. 4.Marshall, Gunnison and Luxen, 1940, Proc. Soc. Exp. Biol. Med., 43:672. 5.Nungester, Hood and Warren, 1943, Proc. Soc. Exp. Biol. Med., 52:287. 6.Portwood, 1944, J. Bact., 48:255.Please refer disclaimer Overleaf. 7.MacFaddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams andWilkins, Baltimore. |
| Customized Product Available | No |
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