Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 6.000 |
| Yeast extract | 3.000 |
| Beef extract | 1.500 |
| Agar | 15.000 |
| pH after sterilization | 7.9±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.5 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the Microbiological assay of Framycetin and Kanamycin B
Principle And Interpretation
This medium is used in the assay of commercial preparations of antibiotics, as well as for antibiotics in body fluids, feeds etc. Medium composition is in accordance to the specifications detailed in the IP, FDA (1,2). Peptone, yeast and beef extract provide necessary growth nutrients for the test organims like Bacillus subtilis. This medium provides solidified substratum for growth of organims. The pH-7.9 maintained in this medium- provides optimum growth conditions for Bacillus subtilis (3). This medium is used to prepare the base as well as seed layer in the microbiological assay of antibiotics such as Framycetin and Kanamycin B. To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized test culture can be overlaid. Even distribution of the layer is important.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Medium amber coloured slightly opalescent gel forms in Petri plates.
pH
7.80-8.00
Cultural Response
MM006: Cultural characteristics observed after an incubation at 32-35°C for 5 days.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant | >=70% | Framycetin, Kanamycin B. |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium E |
|---|---|
| SKU | MM006 |
| Application/ Industry | Pharmaceutical |
| Product Type | Regular |
| Physical Form | Powder |
| Regulatory | IP, FDA, CFR |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Indian Pharmacopeia 2010, Ministry of Health and Family welfare, Government of India, New Delhi. 2.Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983 Title 21, Part 436,Subpart D,Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April 1). 3.Stearn and Stearn, J Bacteriol. 193 3.26(1): 37-55. |
| Customized Product Available | No |
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