Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 6.000 |
| Yeast extract | 3.000 |
| Beef extract | 1.500 |
| Agar | 15.000 |
pH after sterilization 6.55±0.05
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.5 grams in 1000 ml cold purified /distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
This medium is commonly used as base agar for microbiological agar diffusion assays for wide variety of antibiotics. These agar diffusion assays can be performed by cylinders, punched-hole or paper disc tests. This medium is prepared according to the specifications detailed in the IP and CFR(1,2).
Peptone, yeast extract and beef extract nitrogenous, vitamins and mineral requirement for the growth of test organims for the test organims. This medium provides solidified substratum for growth of organisms. This medium is widely used to prepare the base layer in the microbiological assay of antibiotics such as bacitracin, novobiocin and penicillin.
To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Amber coloured slightly opalescent gel forms in Petri plates.
pH
6.50-6.60
Cultural Response
MM005: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >=70% | Spiramycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium B |
|---|---|
| SKU | MM005 |
| Application/ Industry | Pharmaceutical |
| Product Type | Regular |
| Physical Form | Powder |
| Regulatory | IP, FDA, CFR |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Indian Pharmacopoeia 2010, Ministry of Health and Family welfare, Government of India, New Delhi. 2.Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983 Title 21, Part 436,Subpart D,Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April 1). |
| Customized Product Available | No |
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