Tricine–SDS-PAGE is commonly used to separate proteins of lower molecular weight. It is composed of 1M Tris, 1M Tricine and 1% SDS and is preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. It helps for low molecular weight proteins to be separated even in low % acrylamide gels.Lower acrylamide concentrations of Tricine gels help in easy transfer of hydrophobic proteins during Western blotting. This buffer is used as a cathode buffer.