20X MES-SDS Running Buffer is formulated for running proteins on Bis-Tris gels only. This buffer is recommended for separating medium- to large-sized proteins.
20X MES-SDS Running Buffer is used for electrophoresis of proteins on Bis-Tris gels only. This buffer is used for resolving protein samples of very small to medium sized proteins in the range of 2-50 Kda. It contains 1M MES, 1M Tris base, 2% SDS and 20mM EDTA. A reducing agent, Sodium bisulphite, has to be added in this buffer before electrophoresis.