Bradford Reagent is formulated for rapid and accurate quantitative estimation of protein samples. Bradford Reagent contains a dye, Coomassie brilliant Blue G-250, which has an absorbance maximum of 465 nm in unbound state. Bradford reagent is mainly used for the quantitative estimation of protein samples by the Bradford assay method. The Bradford protein assay is based upon the formation of complexes between Coomassie Brilliant Blue G-250 and the protein samples in solution.