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Denhardt’s Solution (100X)
Denhardt's Solution (100X) is composed of blocking agents and is named after David T. Denhardt who formulated it in 1966. This solution is used in membrane-based nucleic acid hybridization protocols like Southern and Northern blotting. It has been proved that preincubation of nucleic acid containing filters in this solution prevents nonspecific binding of single-stranded DNA probes as the high-molecular weight polymers saturate the nonspecific binding sites and artificially increases the concentration of available probe.
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Denhardt's Solution (100X) is composed of blocking agents This solution is used in membrane-based nucleic acid hybridization protocols like Southern and Northern blotting. Preincubation of nucleic acid containing filters in this solution prevents nonspecific binding of single-stranded DNA probes. Denhardt's Solution (100X) is composed of 1% Ficoll 400, 1% Polyvinylpyrrolidone (PVP) and 1% Bovine Serum Albumin
| Product Name | Denhardt’s Solution (100X) |
|---|---|
| SKU | ML095 |
| Customized Product Available | No |
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