10X Tris-Glycine Buffer, pH 8.3

10X Tris-Glycine Buffer, pH 8.3 is formulated for use in preparing gel electrophoresis buffer for native PAGE as this buffer does not contain SDS. This buffer is very convenient to use as it has to be only diluted to 1X with distilled water before performing electrophoresis. 10X Tris-Glycine Buffer is also used to prepare Tris-glycine-methanol transfer buffer for blotting of protein samples on the nitrocellulose membranes during western blotting. This 10X concentrated buffer is diluted to 1X with methanol and water to make a solution containing 20% methanol for western transfer procedure. Methanol is required to prevent the gel from swelling during the transfer and enhances the binding of protein samples to nitrocellulose.