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10X Tris-Glycine Buffer, pH 8.3
SKU:
ML056
Availability: In stock
Only %1 left
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$10.00
10X Tris-Glycine Buffer, pH 8.3 is formulated for use in preparing gel electrophoresis buffer for native PAGE as this buffer does not contain SDS. This buffer is very convenient to use as it has to be only diluted to 1X with distilled water before performing electrophoresis. 10X Tris-Glycine Buffer is also used to prepare Tris-glycine-methanol transfer buffer for blotting of protein samples on the nitrocellulose membranes during western blotting. This 10X concentrated buffer is diluted to 1X with methanol and water to make a solution containing 20% methanol for western transfer procedure. Methanol is required to prevent the gel from swelling during the transfer and enhances the binding of protein samples to nitrocellulose. 10X Tris-Glycine Buffer, pH 8.3 provides a concentrated electrolyte solution for the separation of proteins under non denaturing conditions. This electrophoresis buffer supports native gel running and molecular protein research testing of functional enzyme complexes.
Product Identifier
| Product Name | 10X Tris-Glycine Buffer, pH 8.3 |
|---|---|
| Product Code | ML056-200ML, ML056-1L |
| Kit Packing | 200ml, 1lt |
Introduction
10X Tris-Glycine Buffer, pH 8.3 is commonly used as a electrophoresis buffer in native polyacrylamide gel electrophoresis. This buffer is also used to prepare Tris-glycine-methanol transfer buffer during western blotting procedure. This buffer is supplied as a 10X stock and should be diluted to 1X before use.
Description
10X Tris-Glycine Buffer, pH 8.3 is formulated for use in preparing gel electrophoresis buffer for native PAGE as this buffer does not contain SDS. This buffer is very convenient to use as it has to be only diluted to 1X with distilled water before performing electrophoresis. 10X Tris-Glycine Buffer is also used to prepare Tris-glycine-methanol transfer buffer for blotting of protein samples on the nitrocellulose membranes during western blotting. This 10X concentrated buffer is diluted to 1X with methanol and water to make a solution containing 20% methanol for western transfer procedure. Methanol is required to prevent the gel from swelling during the transfer and enhances the binding of protein samples to nitrocellulose.
Application
10X Tris-Glycine Buffer, pH 8.3 is used in native polyacrylamide gel electrophoresis of proteins and during the electrotransfer of protein samples form the polyacrylamide gels to the nitrocellulose membrane.
Composition
10X Tris-Glycine Buffer, pH 8.3 is composed of 0.25 M Tris and 1.92 M Glycine and the pH is adjusted to 8.3.
Properties
| Appearance | Colorless solution |
|---|---|
| Clarity | Clear and free of particles |
| pH | 8.2-8.4 |
| DNase & RNase | None detected |
| Sterility | No Bacterial or Fungal growth observed after 14 days of incubation as per USP Specifications |
| Suitability test | This solution has been tested and is suitable for use for native electrophoresis and transfer of protein samples from acrylamide gel to nitrocellulose membrane. |
Storage conditions
10X Tris-Glycine Buffer, pH 8.3 has to be stored at 15-25° C. Under recommended condition, the reagent is stable for 12 months.
Warning and Precautions
Not for Medicinal Use. Read the SDS carefully before beginning the protocol. Tris buffer may causes irritation to eyes and skin. Wear protective gloves/protective clothing/eye protection/face protection. Follow good clinical laboratory practices while handling clinical samples. Standard precautions should be followed as per established guidelines. Safety guidelines may be referred in safety data sheets of the product.
Performance and Evaluation
Performance of the solution is expected when the solution is stored at recommended temperature and within the expiry period.
Safety Information
The 10X Tris-Glycine Buffer, pH 8.3 is for laboratory use only, not for drug, household or other uses. Take appropriate laboratory safety measures and wear gloves and safety goggles when handling. Not compatible with disinfecting agents containing bleach. Please refer the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
Technical Assistance
At HiMedia we pride ourselves on the quality and availability of our technical support. For any kind of technical assistance, mail at mb@himedialabs.com.
| Product Name | 10X Tris-Glycine Buffer, pH 8.3 |
|---|---|
| SKU | ML056 |
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