Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Liquid Transport Medium with Swabs#4fa25a
Intended Use
With 2.0 ml Medium recommended for collection & transport of enteric organisms from faecal specimens.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Disodium hydrogen phosphate | 1.100 |
| Sodium thioglycollate | 1.500 |
| Sodium chloride | 5.000 |
| Agar | 5.000 |
| Final pH (at 25°C) | 8.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Remove cap from the tube. Collect sample using capped swab. Discard cap of the tube, replace with capped swab.
Principle And Interpretation
Collect sample or specimen using capped swab. Discard cap of the tube with medium and and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. Process the sample as soon as possible to obtain optimum recovery. In case where immediate recovery (i.e within 2 hours) is not possible, specimens can be stored at 2-25°C and processed within 48 hours.
Type of specimen
Clinical samples- Faeces
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro Diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
Users are recommended to validate the medium for any specific microorganisms other than mentioned in the certificate of analysis based on users unique requirement, as each lot has been tested for the organisms specified on the certificate of analysis.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Cary-Blair medium in tubes. Sterile swab for collection of specimen.
Colour
Clear to slightly opalescent colourless liquid medium in tubes.
Quantity of Medium
2ml of medium in tubes
pH
8.20-8.60
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.
Sterility Check
Passes release criteria
Cultural Response
| Organism | Recovery |
|---|---|
| Klebsiella aerogenes ATCC 13048 (00175*) | Good - Luxuriant |
| Escherichia coli ATCC 25922 (00013*) | Good - Luxuriant |
| Klebsiella pneumoniae ATCC 13883 (00097*) | Good - Luxuriant |
| Neisseria meningitidis ATCC 13090 | Good - Luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | Good - Luxuriant |
| Shigella flexneri ATCC 12022 (00126*) | Good - Luxuriant |
| Vibrio parahaemolyticus ATCC 11344 | Good - Luxuriant |
| Vibrio cholerae ATCC 15748 | Good - Luxuriant |
Storage and Shelf Life
Store between 5-30°C with caps firmly screwed. DO NOT FREEZE. Use before expiry date on label.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiCulture™ Transport swabs w/Cary Blair Medium (A) |
|---|---|
| SKU | MS202A |
| Customized Product Available | No |
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