Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium H
Used for the microbiological assay of Teicoplanin using Bacillus subtilis as a test organism in accordance with European Pharmacopoeia.
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Intended Use
Recommended for turbidimetric assay of Teicoplanin using Bacillus subtilis as a test organism in accordance with EP.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| HM peptone B | 3.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.9±0.1 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract powder
Directions
Suspend 23.0 grams in 1000 ml R-water/purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates or dispense as desired.
Principle And Interpretation
This medium is formulated in accordance with European Pharmacopoeia (1). This medium is employed in the microbiological assay of Teicoplanin using Bacillus subtilis. Essential nutrients for growth of test organism are provided by Peptone and HM peptone B powder in this medium. Agar provides excellent medium for antibiotic diffusion and gives well defined zones of inhibition. Freshly prepared plates should be preferably used for assaying antibiotics. Test organisms is inoculated in sterile seed agar pre-cooled to 40-45°C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully. One of the critical and important step for obtaining good results is use of appropriate standard culture media.
Type of specimen
Antibiotics as per European Pharmacopoeia
Specimen Collection and Handling
Follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations
- Under certain circumstances, the in vitro results of antibiotic susceptibility may not show the same in vivo.
- Inoculum density may affect the zone size. Heavy inoculum may result in smaller zones or too less inoculum may result in bigger zones.
- Freshly prepared plates should be used for antibiotic assays.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance:
Cream to yellow homogeneous free flowing powder
Gelling:
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium:
Light yellow coloured clear to slightly opalescent gel forms in Petri plates
pH:
pH of 2.8% w/v aqueous solution at 25°C.
7.80-8.00
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | >=70% | Teicoplanin |
Key: *Corresponding WDCM numbers.
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and use freshly prepared medium. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Antibiotic Assay Medium H |
|---|---|
| SKU | ME1665 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.European Pharmacopoeia, 2019, European Department for the Quality of Medicines. |
| Customized Product Available | No |
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