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Antibiotic Assay Medium E
For microbiological assay of Neomycin sulphate and Framycetin sulphate using Bacillus subtilis and Bacillus pumilus in accordance with European Pharmacopoeia.
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Intended Use
For microbiological assay of Framycetin Sulphate and Neomycin sulphate using Bacillus subtilis and/or Bacillus pumilus in accordance with European Pharmacopoeia.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 5.000 |
| Disodium hydrogen phosphate, 12H₂O | 26.900 |
| Meat extract | 3.000 |
| Agar | 10.000 |
| pH after sterilization | 7.9±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.67 grams of dehydrated medium powder in 1000 ml R-water/ purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Adjust the pH of the medium, using freshly prepared buffer solution as recommended by the European pharmacopoeia for the antibiotic assayed.
Advice: Recommended for the microbiological assay of Framycetin Sulphate and Neomycin sulphate
Principle And Interpretation
This medium is formulated in accordance to European Pharmacopoeia (1).This medium is widely used for as seed agar in plate assay of Framycetin sulphate and Neomycin sulphate using Bacillus subtilis and/or Bacillus pumilus as test organism.
Peptone and meat extract supply nutrients essential for microbial growth. Phosphates are incorporated in the medium to provide good buffering action. The low concentration of agar facilitates proper diffusion of antibiotic in the seed agar.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45°C and spread evenly over the surface of solidified base agar. Zones of inhibition around the antibiotic are then measured. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for good results.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.0% Agar gel.
Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates.
pH: 7.80-8.00
Cultural Response
ME1347: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bacillus pumilus NCTC 8241 | 50-100 | luxuriant | >=70% | Framycetin sulphate, Neomycin sulphate |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >=70% | Neomycin sulphate and Framycetin sulphate |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium E |
|---|---|
| SKU | ME1347 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. British Pharmacopoeia, 2009, The Stationery office British Pharmacopoeia |
| Customized Product Available | No |
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