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Antibiotic Assay Medium B
For microbiological assay of colistumethate sodium using Bordetella bronchiseptica ATCC 4617 & Escherichia coli ATCC 10536.
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Antibiotic Assay Medium B is used for the microbiological assay of Colistimethate sodium using Bordetella bronchiseptica ATCC 4617 and Escherichia coli ATCC 10536 in accordance with European Pharmacopoeia.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Papaic digest of soyabean | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Glucose monohydrate | 2.500 |
| Agar | 15.000 |
| pH after sterilization | 7.3±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 44.77 grams of dehydrated medium powder in 1000 ml R-water/purified/distilled water with 10 ml polysorbate 80. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring into sterile Petriplates. Adjust the pH of the medium, using freshly prepared buffer solution as recommended by the European pharmacopoeia for the antibiotic assayed.
Principle And Interpretation
Antibiotic Assay Medium B is prepared according to European Pharmacopoeia (1) and is recommended for the assay of Colistimethate sodium using Bordetella bronchiseptica and Escherichia coli as the test organism. Combination of pancreatic digest of casein and papaic digest of soyabean provides essential nutrients for the growth of test organisms.Glucose monohydrate provides fermentable source of carbon, and enhances the growth of test organims. Phosphates in the medium enhance buffering action and sodium chloride maintains osmotic equilibrium. Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45°C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for good results.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
pH: 7.20-7.40
Cultural Response
ME1346: Cultural characteristics observed after an incubation at 35-39°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Bordetella bronchiseptica ATCC 4617 | 50-100 | luxuriant | >=70% | Colistimethate sodium |
| Escherichia coli ATCC 10536 | 50-100 | luxuriant | >=70% | Colistimethate sodium |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium B |
|---|---|
| SKU | ME1346 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. British Pharmacopoeia, 2009, British Pharmacopoeia Commission2 European Pharmacopoeia, 2009, European Pharmacopoeia Commission |
| Customized Product Available | No |
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