Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Luria Bertani HiCynth™ Broth, Miller (Miller Luria Bertani HiCynth™ Broth)
Luria Bertani HiCynth™ Broth, Miller used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.
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Intended Use
Luria Bertani HiCynth™ Broth, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.
Composition
| Ingredients | Gms/Litre |
|---|---|
| HiCynth™ Peptone No.2* | 10.000 |
| HiCynth™ Peptone No.5* | 5.000 |
| Sodium chloride | 10.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Luria Bertani HiCynth™ Broth, Miller (1) is a modification of Luria Bertani Broth, Miller which is slightly different with double amount of sodium chloride as compared to original media described by Lennox (2) for cultivation and maintenance of recombinant strains of Escherichia coli. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. HiCynth™ Peptone No.2 and HiCynth™ Peptone No.5 provides nitrogeneous compounds and carbonaceous compounds, amino acids, vitamins and other growth nutrients required for the growth. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Yellow to amber coloured clear solution in tubes
Reaction: Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH: 7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Luria Bertani HiCynth™ Broth, Miller (Miller Luria Bertani HiCynth™ Broth) |
|---|---|
| SKU | MCD1245 |
| Customized Product Available | No |
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