Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.1* | 15.000 |
| HiCynth™ Peptone No.6* | 5.000 |
| Sodium chloride | 5.000 |
| 4-Methylumbelliferyl β-D-Glucuronide (MUG) | 0.100 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 40.1 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
MUG Tryptone Soya HiCynth™ Agar is used for cultivation of fastidious and non-fastidious microorganisms by fluorogenic method. The medium is rich in nutrients, which makes it suitable for cultivating aerobes as well as anaerobes. Tryptone Soya Agar is used as blood agar base as well as a reference medium when testing selective media to measure the degree of inhibition (1, 2). Tryptone Soya HiCynth™ Agar with MUG is same as Tryptone Soya HiCynth™ Agar with the addition of MUG, used to detect the organisms based on fluorescence.
HiCynth™ Peptone No.1 and HiCynth™ Peptone No.6 provides nitrogenous and carbanaceous compounds, long chain amino acids, vitamins and other growth nutrients. Organisms like Escherichia coli cleave MUG by the enzyme β-glucuronidase to release 4-methylumbelliferone, a fluorogenic end product which produces a visible green-blue fluorescence under long wave UV light.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.01% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery | Fluorescence (under UV) |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% | positive |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | >=70% | negative |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | >=70% | negative |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant | >=70% | negative |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant | >=70% | negative |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | >=70% | negative |
| Staphylococcus epidermidis ATCC 12228 | 50-100 | luxuriant | >=70% | negative |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% | negative |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% | negative |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | MUG Tryptone Soya HiCynth™ Agar |
|---|---|
| SKU | MCD1195 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Gillies R.R., 1964, J. Hyg. Camb., 62 : 1. |
| Customized Product Available | No |
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