Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Luria Bertani HiCynth™ Agar, Miller (Miller Luria Bertani HiCynth™ Agar)
Recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and
molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Luria Bertani HiCynth™ Agar, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.2* | 10.000 |
| HiCynth™ Peptone No.5* | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and and pour into sterile Petri plates.
Principle And Interpretation
Luria Bertani Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (2) is slightly different with double amount of sodium chloride. Luria Bertani HiCynth™ Agar, Miller is a modification of Luria Bertani Agar, Miller wherein animal based peptones are replaced by chemically defined peptones to avoid BSE/TSE risks associated with animal based peptones. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis, are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.
HiCynth™ Peptone No.2 and HiCynth™ Peptone No.5 provides nitrogeneous and carbanaceous compounds, amino acids, vitamins and other growth factors required for the growth. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Luria Bertani HiCynth™ Agar, Miller (Miller Luria Bertani HiCynth™ Agar) |
|---|---|
| SKU | MCD1151 |
| Customized Product Available | No |
Write Your Own Review