HiMedia's HiQuanti One Step Probe Based RT-PCR Kit is ready to use and can be used with diverse RNA samples and custom primers and probes. The kit uses M-MuLV Reverse Transcriptase and Taq DNA Polymerase, premixed together at concentrations optimized to balance sensitivity and specificity. Owing to its good amplification efficiency, specificity and stability, it can greatly improve the sensitivity. The product is designed for convenient, good sensitivity one step RT-PCR reaction. Its unique enzyme and tailor-made buffer ensures the efficiency and accuracy of RT-PCR reaction.
HiMedia’s Hi-Quanti One Step Probe Based RT-PCR Kit is designed for simplicity and convenience in carrying out probe based reverse transcription polymerase chain reaction (RT-PCR) in a one-tube format. Using a unique combination of enzymes, to ensure high specificity and sensitivity with an optimized buffer system, performing both cDNA synthesis and Real-Time PCR amplification in a single tube using gene-specific primers, probes and target RNAs has become very easy either as uniplex or multiplex diagnostic assay.