HiPurA® XL1-Blue Competent Cells are specially prepared chemically competent cells which makes cloning experiments easier. Competent cells are ready-to-use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily
HiPurA® XL1-Blue Competent Cells are specially prepared chemically competent cells which makes
cloning experiments easier. Competent cells are ready-to-use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA® XL1- Blue Competent Cells are prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for regular sub cloning of recombinant plasmids. XL1-Blue is the most commonly used E.coli strain for routine cloning applications through blue/white screening of colonies on plates containing X-Gal and IPTG as the lacZ?M15 marker provides ?-complementation of the ?-galactosidase gene. A stock of pUC19 plasmid DNA is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and
detergents