HiScript Two Step RT-PCR Kit

HiScript Two Step RT-PCR Kit is designed for reverse transcription where cDNA (complementary DNA) is synthesized in vitro from an mRNA template by an enzyme that has reverse transcriptase activity followed by PCR amplification. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MuLV RTase) is an RNAdependent DNA polymerase that can be used in cDNA synthesis. This step is very important in order to perform PCR since DNA polymerase can act only on DNA templates. The resulting cDNA is single-stranded and this process is called reverse transcription (RT) or first strand cDNA synthesis. This step is very important in order to perform PCR since DNA polymerase can act only on DNA templates. The resulting cDNA is single-stranded and this process is called reverse transcription (RT) or first strand cDNA synthesis. The kit contains Random Hexamer, Oligo (dT) and Random Hexamer : Oligo(dT) Mix along with Ribonuclease Inhibitor. The HiScript Two Step RT-PCR Kit is designed for simplicity and convenience in carrying out RT-PCR followed by PCR amplification. Owing to its good amplification efficiency, specificity and stability, it can greatly improve the sensitivity. The product is designed for convenient, good sensitivity for two step RT-PCR reaction. Its unique enzyme and tailor-made buffer can ensure the efficiency and accuracy of reverse transcription PCR reaction.