Quick and easy single-tube reaction; suitable for a variety of RNA templates, without optimizing the
reaction conditions,an unique combination of enzymes, to ensure high specificity and sensitivity of the response,an optimized buffer system ensures efficient reverse transcription and amplification process
The HiScript One Step RT-PCR Kit is designed for simplicity and convenience in carrying out RT-PCR in a one-tube format. Using this convenient one-step formulation, performing both cDNA synthesis and
PCR amplification in a single tube using gene-specific primers and target RNAs has become very easy. The One-Step RT-PCR Kit comes complete and ready-to-use for RT-PCR. The kit can be used with
diverse RNA samples and custom primers. The kit uses M-MuLV Reverse Transcriptase and Taq DNA
Polymerase, premixed together at concentrations optimized to balance sensitivity and specificity.
Owing to its good amplification efficiency, specificity and stability, it can greatly improve the
sensitivity. The product is designed for convenient, good sensitivity one step RT-PCR reaction. Its
unique enzyme and tailor-made buffer can ensure the efficiency and accuracy of reverse transcription
PCR reaction.