Hi‐PCR® Colistin Resistance Encoding Gene Quantification Probe PCR Kit is designed to detect mcr‐1 gene in FAM channel with Internal Control in JOE channel in a single tube reaction. The kit allows sensitive and specific detection of mcr‐1 in a single tube reaction. The kit also allows absolute quantification of the mcr‐1 gene, based on the standard curve created using standards of known copy number.
Colistin have recently regained popularity for treatment of severe infections caused by extensively drug-resistant (XDR) Gram-negative bacterial strains. As a likely consequence, emergence of Colistin resistance is being increasingly reported in the clinical setting, especially among carbapenem-resistant Klebsiella pneumoniae isolates. Acquired resistance to polymyxins is generally associated with chromosomal mutations. However, a new plasmid-mediated transferable resistance determinant, the mcr-1 gene has been described recently. Hi-PCR® Colistin Resistance Encoding Gene Quantification Probe PCR Kit is designed for sensitive and specific detection of mcr-1 gene with Internal Control in a single tube reaction. The kit also allows absolute quantification of the mcr-1 gene, based on the standard curve created using standards of known copy number.