Clinical microbiology laboratory is responsible for the isolation or detection of microorganisms to establish the diagnosis of infection. Microorganisms are typically identified by morphological, physiological, and biochemical characteristics, among other attributes. However, procedures for characterizing these features are time-consuming and sometimes yield incorrect or no results. DNA analysis has been increasingly used for microorganism identification. The analysis of the nucleotide sequences of the 16S ribosomal RNA gene has emerged as the single best method to identify bacteria. Hi-PCR® 16S rRNA Semi-Q PCR Kit is designed for the detection of a specific sequence of the 16S rRNA gene in bacteria from various sources.