HiMedia's Hi‐PCR® E. coli O157:H7 Semi‐Q PCR Kit is a qualitative conventional PCR kit which allows the amplification eaeA gene (450 bp) using specific primers. The amplified target is detected by using agarose gel electrophoresis. This kit also contains Internal control and Positive control.
E.coli O157:H7 is an aerobic bacterium that causes hemolytic colitis and hemolytic uremic syndrome. E. coli O157:H7 is also known to produce Shiga-like toxins which cause severe symptoms. The main mode of infection is through food, untreated water and unpasteurized milk. This organism is characterized by Endotoxin shock as one of the lethal manifestation of infection. The Hi-PCR® E. coli O157:H7 Semi-Q PCR Kit is designed for detection of specific sequence for E. coli O157:H7 in various food sources, cells, environmental and clinical samples. Conventional PCR testing can provide rapid, sensitive and specific detection of E.coli O157:H7.