Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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HiPurA® Yeast RNA Purification Kit
HiPurA® Yeast RNA Purification Kit provide a fast and easy method for purification of total RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT?PCR, Differential display, Expression?array and expression?chip analysis
and cDNA library construction. The RNA purification procedure using the miniprep spin-columns comprises of three steps viz, adsorption of RNA to the membrane, removal of residual contaminants and elution of pure RNA.
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This kit simplifies isolation of yeast RNA with spin-column procedure. The lysis buffer provided
in the kit helps in cell disruption and denaturation, samples are centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the HiElute Miniprep spin-column (Capped) membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. high-quality RNA is finally eluted in the Elution Solution provided with the kit. This kit yields up to 90 µg of total RNA from 30 million yeast cells.
| Product Name | HiPurA® Yeast RNA Purification Kit |
|---|---|
| SKU | MB611 |
| Customized Product Available | No |
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