HiPurA® DNA/ RNA Purification Kit

This kit simplifies isolation of RNA from animal cells, animal tissues and for cleanup of RNA from crude enzymatic reactions (e.g. DNase digestion, Proteinase digestion, RNA ligation, and labeling reaction) with spin-column procedure. The lysis buffer provided in the kit helps in cell disruption and denaturation, samples are centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material.Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the HiElute Miniprep spin-column (Capped) membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. high-quality RNA is finally eluted in the Elution Solution provided with the kit. A specialized high?salt buffer system allows upto 100 g of total RNA longer than 200 bases to bind to the HiElute Miniprep spin-column (Capped) membrane.