Agarose gel electrophoresis is a method used to separate DNA and RNA molecules according to their
molecular size. This is achieved when negatively charged nucleic acids migrate through an agarose gel
matrix under the influence of an electric field (electrophoresis). Shorter molecules move faster and
migrate farther than the larger ones.
Agarose gel electrophoresis is a method used to separate DNA and RNA molecules according to their
molecular size. This is achieved when negatively charged nucleic acids migrate through an agarose gel matrix under the influence of an electric field (electrophoresis). Shorter molecules move faster and migrate farther than the larger ones. This method issimple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated by other procedures such as density gradient centrifugation. The position of DNA in the agarose gel is visualized by staining with low concentration of fluorescent intercalating dyes, such as Ethidium bromide.