HiPurA® 96 PCR Product Purification Kit

The HiPurA® 96 PCR Product Purification Kit provides a simple, rapid method for purifying DNA (100 bp-10 kb) from PCR and other enzymatic reactions by the use of silica binding in a 96-well format. It is based on the advanced silica binding principle presented in a centrifugation and vacuum format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. DNA binds specifically to the silica-gel membrane while contaminants such as free nucleotides, primer dimers (upto 50 bp), proteins, MgCl2, mineral oil, etc. pass through and are completely removed in wash step. The clean, concentrated DNA is eluted in a small volume of low salt buffer. Concentration of recovered DNA can be estimated by agarose gel electrophoresis. The PCR product recovery is 80-95% depending on the size of DNA from 25-100µl fo PCR product.