Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Tryptone Soya Agar w/ Magnesium Sulphate is recommended for cultivation of coliforms in accordance with AOAC.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 15.000 |
| Peptic digest of animal tissue | 5.000 |
| Sodium chloride | 5.000 |
| Magnesium sulphate | 1.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 41.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
The faecal coliform group is restricted to organisms that grow in the gastrointestinal tract of human and warm-blooded animals. It includes members of at least three genera : Escherichia , Klebsiella and Enterobacter
Tryptone Soya Agar with Magnesium Sulphate (TSAM) is used for the enumeration of coliform bacteria by hydrophobic grid membrane filter (HGMF) (1). The membrane filter is imprinted with hydrophobic material in grid pattern. The hydrophobic lines act as barriers to spread the colonies, thereby dividing membrane filter surface into separate compartments of equal and known size. HGMF membrane filters has pore size of 0.45 mm.
Casein enzymic hydrolysate and peptic digest of animal tissue provide the necessary nutrients to the organisms. Sodium chloride maintains osmotic equilibrium while magnesium sulphate enhances maximum recovery of the coliform bacteria on membrane filters. For E.coli / coliform counts place HGMF on surface of pre-dried Tryptone Soya Agar with Magnesium Sulphate. Incubate at 25°C for 4-5 hours for dry foods and 4-5 hrs at 35°C for all other foods. The membrane is placed on the surface of other differential / selective medium after pre-incubation on TSAM, to enable detection of coliforms (2, 3).
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 4.15% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Cultural Response
M990: Cultural characteristics observed after an incubation at different temperatures for 22-24 hours.
| Organism | Inoculum (CFU) | Growth at 35-37°C | Growth at 44-46°C |
|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | luxuriant |
| Enterococcus faecalis ATCC 29212 | 50-100 | luxuriant | inhibited |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | inhibited |
| Shigella flexneri ATCC 12022 | 50-100 | luxuriant | inhibited |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Official Methods of Analysis of AOAC International 16th Edition, Vol. 1, 1995.
- J.AOAC, 1983, 66:897.
- J.AOAC, 1984, 67:812.
| Product Name | Tryptone Soya Agar w/ Magnesium Sulphate (TSAM) |
|---|---|
| SKU | M990 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Official Methods of Analysis of AOAC International 16th Edition, Vol. 1, 1995.2.J.AOAC, 1983, 66:897.3.J.AOAC, 1984, 67:812. |
| Customized Product Available | No |
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