Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for promoting the sporulation of yeasts.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 10.000 |
| Dextrose | 1.000 |
| Sodium chloride | 5.000 |
| Agar | 20.000 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 36 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour in sterile Petri plates.
Principle and Interpretation
The medium contains Peptic digest of animal tissue which provides nitrogen and other nutrients necessary to support bacterial growth. Dextrose is the source of carbohydrate. Monopotassium phosphate buffers the media. Magnesium sulphate is a source of divalent cations. Sodium chloride is an essential ion and helps in maintaining the osmotic balance of the medium. Agar is the solidifying agent.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates.
Cultural Response: M983: Cultural characteristics observed after an incubation at 25-30°C for 4-5 days.
| Organism | Growth |
|---|---|
| *Aspergillus brasiliensis ATCC 16404 | luxuriant |
| Candida albicans ATCC 10231 | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Ivor H Evans. Yeast Protocols: Methods in Cell and Molecular Biology. Methods in Molecular Biology. Humana Press. Totowa, New Jersey.
| Product Name | Modified Gorodkowa Agar |
|---|---|
| SKU | M983 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Ivor H Evans. Yeast Protocols: Methods in Cell and Molecular Biology. Methods in Molecular Biology. Humana Press.Totowa, New Jersey. |
| Customized Product Available | No |
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